Overview

  • Dr. X. Nancy Xu, Professor in Chemistry and Biochemistry and affiliated faculty member of Biomedical Engineering (affiliated), is the AAAS Fellow and recipient of Nano 50TM Innovator Award and ODU Faculty Research Achievement Award.
  • Selected Publications

    Articles In Academic Journals

    Year Title
    2018 Single Gold Nanoparticle Plasmonic Spectroscopy for Study of Chemical-Dependent Efflux Function of Single ABC Transporters of Single Live Bacillus subtilis CellsAnalyst.
    2017 Probing of effects of silver nanoparticles on single liver tumor cellsPapers of the American Chemical Society.
    2016 Single Nanoparticle Plasmonic Spectroscopy for Study of Charge-Dependent Efflux Function of Multidrug ABC Transporters of Single Live Bacillus subtilis CellsThe Journal of Physical Chemistry C. 21007.
    2015 Wavelength dependent specific plasmon resonance coupling of single silver nanoparticles with EGFPNanoscale. 17623-17630.
    2014 Design and study of efflux function of EFGP fused MexAB-OprM membrane transporter in Pseudomonas aeruginosaAnalyst. 3088-3096.
    2014 Design and study of the efflux function of the EGFP fused MexAB-OprM membrane transporter in Pseudomonas aeruginosa using fluorescence spectroscopyAnalyst. 3088-3096.
    2013 Real-time in vivo imaging of size-dependent transport and toxicity of gold nanoparticles in zebrafish embryos using single nanoparticle plasmonic spectroscopyInterface Focus. 20120098.
    2013 Silver nanoparticles incite size-and dose-dependent developmental phenotypes and nanotoxicity in zebrafish embryosChemical research in toxicology. 1503-1513.
    2013 Silver nanoparticles induce developmental stage-specific embryonic phenotypes in zebrafishNanoscale. 11625-11636.
    2013 Study of charge-dependent transport and toxicity of peptide-functionalized silver nanoparticles using zebrafish embryos and single nanoparticle plasmonic spectroscopyChemical research in toxicology. 904-917.
    2013 Ultrasensitive analysis of binding affinity of HIV receptor and neutralizing antibodies using solution-phase electrochemiluminescence assayJournal of Electroanalytical Chemistry. 53-60.
    2012 Far-field photostable optical nanoscopy (PHOTON) for real-time super-resolution single-molecular imaging of signaling pathways of single live cellsNanoscale. 2797-2812.
    2012 High-throughput ultrasensitive characterization of chemical, structural and plasmonic properties of EBL-fabricated single silver nanoparticlesNanoscale. 380-385.
    2012 In vivo quantitative study of sized-dependent transport and toxicity of single silver nanoparticles using zebrafish embryosChemical research in toxicology. 1029-1046.
    2012 Single nanoparticle spectroscopy for real-time in vivo quantitative analysis of transport and toxicity of single nanoparticles in single embryosAnalyst. 2973-2986.
    2011 Design and probing of efflux functions of EGFP fused ABC membrane transporters in live cells using fluorescence spectroscopyAnalytical and bioanalytical chemistry. 223-235.
    2011 Localized surface plasmon resonance of single silver nanoparticles studied by dark-field optical microscopy and spectroscopyJournal of Applied Physics. 034310.
    2011 Multicolored nanometre-resolution mapping of single protein–ligand binding complexes using far-field photostable optical nanoscopy (PHOTON)Nanoscale. 3567-3572.
    2010 Design and characterization of optical nanorulers of single nanoparticles using optical microscopy and spectroscopyNanoscale. 1715-1722.
    2010 Electric pulses to prepare feeder cells for sustaining and culturing of undifferentiated embryonic stem cellsBiotechnology journal. 588-595.
    2010 Probing of multidrug ABC membrane transporters of single living cells using single plasmonic nanoparticle optical probesAnalytical and bioanalytical chemistry. 3317-3328.
    2010 Study of cytotoxic and therapeutic effects of stable and purified silver nanoparticles on tumor cellsNanoscale. 942-952.
    2010 Synthesis and characterization of tunable rainbow colored colloidal silver nanoparticles using single-nanoparticle plasmonic microscopy and spectroscopyJournal of materials chemistry. 9867-9876.
    2009 Correlation and Characterization of Three-Dimensional Morphologically Dependent Localized Surface Plasmon Resonance Spectra of Single Silver Nanoparticles Using Dark-Field Optical Microscopy and Spectroscopy and Atomic Force MicroscopyThe Journal of Physical Chemistry C. 74-81.
    2009 Random walk of single gold nanoparticles in zebrafish embryos leading to stochastic toxic effects on embryonic developmentsNanoscale. 138-152.
    2009 Real-time imaging and tuning subcellular structures and membrane transport kinetics of single live cells at nanosecond regimeThe Journal of Physical Chemistry B. 14393-14404.
    2008 Design of stable and uniform single nanoparticle photonics for in vivo dynamics imaging of nanoenvironments of zebrafish embryonic fluidsACS Nano. 1371-1380.
    2008 Photostable single-molecule nanoparticle optical biosensors for real-time sensing of single cytokine molecules and their binding reactionsJournal of the American Chemical Society. 17095-17105.
    2007 ACS Nano 2007, 1, 133–143CrossRef| PubMed| CAS| Web of Science® Times Cited.
    2007 Design and synthesis of single-nanoparticle optical biosensors for imaging and characterization of single receptor molecules on single living cellsAnalytical chemistry. 7708-7718.
    2007 Electrochemiluminescence detection in bioanalysisChemical Analysis-New York-Interscience then John Wiley-. 235.
    2007 In vivo imaging of transport and biocompatibility of single silver nanoparticles in early development of zebrafish embryosACS Nano. 133-143.
    2007 Probing membrane transport of single live cells using single-molecule detection and single nanoparticle assayChemical Analysis-New York-Interscience then John Wiley-. 41.
    2005 Imaging and assembly of nanoparticles in biological systemsHandbook of nanostructured biomaterials and their applications in nanobiotechnology. 435-456.
    2004 Real-time probing of membrane transport in living microbial cells using single nanoparticle optics and living cell imagingBiochemistry. 10400-10413.
    2004 Single live cell imaging of chromosomes in chloramphenicol-induced filamentous Pseudomonas aeruginosaBiochemistry. 175-182.
    2004 Size and Temperature Dependence of Surface Plasmon Absorption of Gold Nanoparticles Induced by Tris (2, 2 ‘-bipyridine) ruthenium (II)The Journal of Physical Chemistry B. 15543-15551.
    2004 Using nanoparticle optics assay for direct observation of the function of antimicrobial agents in single live bacterial cellsBiochemistry. 140-147.
    2003 Direct observation of substrate induction of resistance mechanism in Pseudomonas aeruginosa using single live cell imagingBiochemical and biophysical research communications. 941-949.
    2003 Real-time measurements of single membrane pump efficiency of single living Pseudomonas aeruginosa cells using fluorescence microscopy and spectroscopyBiochem. Biophys. Res. Commun. 79-86.
    2003 Single live cell imaging of chromosomes in chloramphenicol-induced filamentous Pseudomonas aeruginosaBiochemistry. 175-182.
    2003 Single-molecule detection of efflux pump machinery in Pseudomonas aeruginosaBiochem. Biophys. Res. Commun.. 79.
    2002 Direct measurement of sizes and dynamics of single living membrane transporters using nanoopticsNano Letters. 175-182.
    2002 Single live cell imaging for real-time monitoring of resistance mechanism in Pseudomonas aeruginosaJournal of biomedical optics. 576-586.
    2001 Novel solution-phase immunoassays for molecular analysis of tumor markersAnalyst. 1285-1292.
    1998 Long-range electrostatic trapping of single-protein molecules at a liquid-solid interfaceScience. 1650-1653.
    1997 Direct measurement of single-molecule diffusion and photodecomposition in free solutionScience. 1106-1109.
    1996 Generation by Electron Transfer of an Emitting State Not Observed by Photoexcitation in a Linked Ru (bpy) 32+- Methyl ViologenJournal of the American Chemical Society. 3656-3660.
    1995 Immobilization and hybridization of DNA on an aluminum (III) alkanebisphosphonate thin film with electrogenerated chemiluminescent detectionJournal of the American Chemical Society. 2627-2631.
    1994 Electrogenerated chemiluminescence. 55. Emission from adsorbed Ru (bpy) 32+ on graphite, platinum, and goldLangmuir. 2409-2414.
    1994 Immobilization of DNA on an aluminum (III) alkanebisphosphonate thin film with electrogenerated chemiluminescent detectionJournal of the American Chemical Society. 8386-8387.
    1993 The Electrochemistry of Tin in the Aluminum Chloride-1-methyl-3-ethylimidazolium Chloride Molten SaltJournal of The Electrochemical Society. 618-626.
    1992 Electrodeposition of Silver on Metallic and Nonmetallic Electrodes from the Acidic Aluminum Chloride-1-Methyl-3-Ethylimidazolium Chloride Molten SaltJournal of The Electrochemical Society. 1295-1300.
    1992 Electrodeposition of metals from room-temperature chloroaluminate molten saltsProc. of Electrochem. Soc.. 445.
    1992 The Electrochemistry of Gold at Glassy Carbon in the Basic Aluminum Chloride-1-Methyl-3-ethylimidazolium Chloride Molten SaltJournal of The Electrochemical Society. 3103-3108.
    1991 Electrodissolution and electrodeposition of lead in an acidic room temperature chloroaluminate molten saltJournal of The Electrochemical Society. 1886-1890.

    Chapters

    Year Title
    2017 Far-Field Photostable Optical Nanoscopy (PHOTON)Encyclopedia of Spectroscopy and Spectrometry. Oxford: Elsevier.
    2007 Outlooks of ultrasensitive detection in bioanalysisNew Frontiers in Ultrasensitive Bioanalysis: Advanced Analytical Chemistry Applications in Nanobiotechnology, Single Molecule Detection, and Single Cell Analysis. Wiley.
    2004 Nanoparticles for Live-Cell DynamicsEncyclopedia of Nanoscience and Nanotechnology. American Scientific Publishers.

    Conference Papers

    Year Title
    2011 Design of in vivo assays for probing of physiochemical dependent biocompatibility and toxicity of naomaterials
    2011 Plasmonic nanoparticle probes for study of single ABC multidrug membrane transporters of single live cells
    2011 Single molecule plasmonic nanoparticle biosensors for molecular imaging of single live cells
    2011 Super-resolution and single molecule imaging of SERS hot spots
    2010 Design of photostable single molecule nanoparticle biosensors for real time probing single living cells
    2009 Photostable single molecule nanoparticle optical biosensors for sensing and imaging of single protein molecules and their binding kinetics
    2008 BIOT 403-Developing photostable and biocompatible single nanoparticle probes for in vivo imaging of early development of zebrafish embryos
    2008 Developing Single Nanoparticle Optics and in Vivo Assays for Real-Time Characterization of Transport and Biocompatibility of Nanomaterials.  2435-2435.
    2007 ANYL 382-Design of single nanoparticle optical sensors for imaging single receptor molecules on single living cells
    2006 NIRT (II): Design of biocompatible nanoparticles for probing living cellular functions and their potential environmental impacts
    2005 NIRT (I): Design of biocompatible nanoparticles for probing living cellular functions and their potential environmental impacts
    2005 Real-time study of signal transduction pathways using single nanoparticle optics and single living cell imaging.  U259-U259.
    2005 Single nanoparticle optics assay for sensing single protein molecules on single living cells.  U201-U202.
    2005 Study of cytotoxicity of nanomaterials in single living cells.  U248-U248.
    2002 Electrochemilumiescence study of affinity, size and conformation of proteins..  U650-U650.
    2001 Single-molecule dynamics and interactions at living interfaces..  U228-U228.
    2000 Molecular analysis of biomarkers for earlier cancer detection.  15-26.
    1999 Combining spectroscopy and chromatography in analytical chemistry..  U161-U161.
    1997 Real-time monitoring of single-molecule reactions in aqueous solution..  108-ANYL.
    1996 Analytical instrumentation in the fourth dimension..  104-ANYL.

    Research Overview

  • Dr. Xu has directed NSF and NIH funded nanoscale interdisciplinary research programs, aiming to develop and apply cutting-edge bionanotechnologies and ultrasensitive detection tools to address most challenging questions in biomedical and nano sciences. Her research program lies at the interface of Chemistry, Biology and Engineering as described at http://ww2.odu.edu/~xhxu/research.html
  • Grants

  • New Tools for Real-Time Single Molecular Imaging of ABC Transporter  awarded by NIH 2018 - 2023
  • New tools for real-time single molecule probing of chromatin dynamics and functions in single live cells  awarded by NSF 2018 - 2023
  • EFRI-CEE Preliminary Proposal: New Tools for Real-Time Single Molecule Probing of Chromatin Dynamics and Functions in Single Live Cells In Vivo  awarded by NSF 2018 - 2022
  • Research Supplement to Promote Diversity in Health-Related Research  awarded by NIH 2017 - 2019
  • New Photostable Nanoprobes for Real-time Imaging of Single Live Cells  awarded by NIH 2016 - 2019
  • Photostable Multiplexing Nanoassays For Real-Time Study of Embryonic STEM Cells  awarded by US Public Health Service (NIH) 2016 - 2018
  • Photostable Multiplexing NanoAssays for Real-Time Study of Embryonic Stem Cells  awarded by NIH 2015 - 2018
  • Catalyzing New International Collaborations (CNIC) Supplement  awarded by NSF (CBET 0507036) 2014 - 2018
  • GRA Supplement  awarded by NSF (CBET 0507036) 2014 - 2018
  • NSF BRAIN EAGER: New Tools for Real-Time Imaging of Molecular-Resolution Connectomics of Synapses  awarded by National Science Foundation 2014 - 2018
  • REU Supplement  awarded by NSF (CBET 0507036) 2014 - 2018
  • Catalyzing New International Collaborations (CNIC) Supplement  awarded by NSF (CBET 0507036) 2013 - 2018
  • NSF/GRA Supplement (CBET 0507036)  awarded by NSF/GRA 2009 - 2018
  • Nanoassay for Real-time Molecular Probing ABC Transporter  awarded by NIH (R01 GM0764401) 2006 - 2014
  • Nanoassay for study of multidrug resistance in bacteria  awarded by National Institutes of Health (3 R01 GM076440-01S1) 2006 - 2013
  • Nanoassay for Real-time Molecular Probing ABC Transporter  awarded by NIH (3 R01 GM076440-01S1) 2009 - 2012
  • NSF/GRA Supplement (CBET 0507036)  awarded by NSF/GRA 2008 - 2009
  • NSF/GRA Supplement (CBET 0507036)  awarded by NSF/GRA 2007 - 2009
  • NSF/GRA Supplement for NIRT (BES 0631940)  awarded by NSF/GRA 2006 - 2009
  • Design of nanoparticles for probing living cellular functions and their potential environmental impacts  awarded by National Science Foundation 2005 - 2009
  • NSF/GRA Research Supplement (BES 0542582)  awarded by NSF/GRA 2005 - 2009
  • NSF/GRA Supplement (BES 0541661)  awarded by NSF/GRA 2005 - 2009
  • NSF/RET Supplement for NIRT (BES 0507036)  awarded by NSF/GRA 2005 - 2009
  • NSF (MRI: DMR 0420304)  awarded by NSF 2004 - 2007
  • MURI-02: Subcellular Responses to Narrowbound and Wideband Radiofrequency Radiation  awarded by Air Force Office of Scientific Research (AFOSR: #F49620-01-1-0320) Multidisciplinary University Research Initiative (MURI) 2002 - 2007
  • Real-Time Molecular Study of Bystander Effect Using Living Cell Imaging and Nanoparticle Optics  awarded by Department of Energy (DE-FG02-03ER63646) 2003 - 2006
  • Real-Time Single-Molecule Chemical Microscopy  awarded by NIH (RR15057-01) 2000 - 2004
  • Expertise Keywords

  • Biotechnology : Single Cell Analysis/Imaging
  • Chemistry : Analytical/Biochemistry/Bionanotechnology/Bionanomaterial/Biomedical Engineering
  • Nanotechnology : Bionanotechnology/Bionanomaterial/Biomedical Engineering
  • Real-time imaging : Single Live Cells/Embryos; Single Molecule/Nanoparticle Imaging
  • Education And Training

  • Ph.D. in Chemistry, University of Mississippi 1992
  • B.S. in Physical Chemistry, Xiamen University 1985
  • Full Name

  • Xiaohong Xu