Selected Publications

Articles In Academic Journals

Year Title
2018 Electropermeabilization by uni- or bipolar nanosecond electric pulses: The impact of extracellular conductivity.Bioelectrochemistry (Amsterdam, Netherlands). 10-19.
2018 Excitation and injury of adult ventricular cardiomyocytes by nano- to millisecond electric shocksScientific Reports. 12.
2017 Activation of the phospholipid scramblase TMEM16F by nanosecond pulsed electric fields (nsPEF) facilitates its diverse cytophysiological effects.The Journal of biological chemistry. 19381-19391.
2017 Activation of the phospholipid scramblase tmem16f by nanosecond pulsed electric field (nspef) facilitates its diverse cytophysiological effectsJournal of Biological Chemistry.
2017 Delayed hypersensitivity to nanosecond pulsed electric field in electroporated cells.Scientific Reports. 10992.
2017 Effect of Cooling On Cell Volume and Viability After Nanoelectroporation.The Journal of Membrane Biology. 217-224.
2017 Electrosensitization Increases Antitumor Effectiveness of Nanosecond Pulsed Electric Fields In Vivo.Technology in Cancer Research & Treatment. 1533034617712397.
2017 Human placenta hydrogel reduces scarring in a rat model of cardiac ischemia and enhances cardiomyocyte and stem cell culturesActa Biomaterialia. 92-104.
2017 Selective susceptibility to nanosecond pulsed electric field (nsPEF) across different human cell types.Cellular and molecular life sciences : CMLS. 1741-1754.
2016 Cell Electrosensitization Exists Only in Certain Electroporation Buffers.PLOS ONE. e0159434.
2016 Electrosensitization assists cell ablation by nanosecond pulsed electric field in 3D cultures.Scientific Reports. 23225.
2016 The cytotoxic synergy of nanosecond electric pulses and low temperature leads to apoptosis.Scientific Reports. 36835.
2015 Diffuse, non-polar electropermeabilization and reduced propidium uptake distinguish the effect of nanosecond electric pulses.Biochimica et biophysica acta. 2118-25.
2015 Ion transport into cells exposed to monopolar and bipolar nanosecond pulses.Bioelectrochemistry (Amsterdam, Netherlands). 44-51.
2015 Multiple nanosecond electric pulses increase the number but not the size of long-lived nanopores in the cell membrane.Biochimica et biophysica acta. 958-66.
2014 Bipolar nanosecond electric pulses are less efficient at electropermeabilization and killing cells than monopolar pulses.Biochemical and biophysical research communications. 568-73.
2014 Calcium-mediated pore expansion and cell death following nanoelectroporation.Biochimica et biophysica acta. 2547-54.
2014 Cancellation of cellular responses to nanoelectroporation by reversing the stimulus polarity.Cellular and molecular life sciences : CMLS. 4431-41.
2014 Disassembly of actin structures by nanosecond pulsed electric field is a downstream effect of cell swelling.Bioelectrochemistry (Amsterdam, Netherlands). 88-95.
2013 Facilitation of electroporative drug uptake and cell killing by electrosensitizationJ Cell Mol Med. 154-9.
2013 Recruitment of the intracellular Ca by ultrashort electric stimuli: The impact of pulse durationCell Calcium. 00083-3.
2013 Recruitment of the intracellular Ca2+ by ultrashort electric stimuli: The impact of pulse durationCell Calcium. 145-150.
2012 Oxidative effects of nanosecond pulsed electric field exposure in cells and cell-free mediaArch Biochem Biophys. 55-64.
2011 Dose-dependent thresholds of 10-ns electric pulse induced plasma membrane disruption and cytotoxicity in multiple cell linesPLOS ONE. 0015642.
2011 Electroporation-induced electrosensitizationPLOS ONE. 0017100.
2011 Manipulation of cell volume and membrane pore comparison following single cell permeabilization with 60- and 600-ns electric pulsesBiochim Biophys Acta. 792-801.
2010 Analysis of plasma membrane integrity by fluorescent detection of Tl(+) uptakeJ Membr Biol. 15-26.
2010 Crystallization of community-acquired respiratory distress syndrome toxin from Mycoplasma pneumoniaeActa Crystallographica Section F-Structural Biology and Crystallization Communications. 294-296.
2010 Nanopore Formation in Cells Exposed to Nanosecond Electric PulsesBiophysical Journal. 375a-375a.
2010 Selective cytotoxicity of intense nanosecond-duration electric pulses in mammalian cellsBiochim Biophys Acta. 1210-9.
2009 Lipid nanopores can form a stable, ion channel-like conduction pathway in cell membraneBiochem Biophys Res Commun. 181-6.
2009 Nanosecond-Duration Electric Pulses Open Nanometer-Size Pores in Cell Plasma MembraneWorld Congress on Medical Physics and Biomedical Engineering, Vol 25, Pt 13. 17-20.
2008 A-Fulgidus SRP54 m-domainJournal of biomolecular NMR. 241-248.
2007 Membrane permeabilization and cell damage by ultrashort electric field shocksArch Biochem Biophys. 109-18.
2006 Oxygen enhances lethal effect of high-intensity, ultrashort electrical pulsesBioelectromagnetics. 221-5.
2005 Assembly of T beta RI : T beta RII : TGF beta ternary complex in vitro with receptor extracellular domains is cooperative and isoform-dependentJournal of molecular biology. 1052-1068.
2002 Solution structure of protein SRP19 of Archaeoglobus fulgidus signal recognition particleJournal of molecular biology. 145-158.
2001 Complexes with truncated RNAs from the large domain of Archaeoglobus fulgidus signal recognition particleFems Microbiology Letters. 105-110.
2001 Sequence-specific H-1, C-13 and N-15 signal assignments and secondary structure of Archaeoglobus fulgidus SRP19Journal of biomolecular NMR. 187-188.
1999 In vitro assembly of yeast 5S rRNA and a fusion protein containing ribosomal protein L5 and maltose binding proteinBiochimie. 1015-1023.
1998 Current state and implications of research on biological effects of millimeter waves: a review of the literatureBioelectromagnetics. 393-413.
1998 Ultra-wide band electromagnetic radiation does not affect UV-induced recombination and mutagenesis in yeastBioelectromagnetics. 128-130.
1997 Effect of millimeter waves on UV-induced recombination and mutagenesis in yeastBioelectrochemistry and Bioenergetics. 227-232.
1997 Lack of genetic effects of ultrawide-band electromagnetic radiation in yeastElectro- and Magnetobiology. 195-201.
1987 Contribution of a Photoreactivable Component to the Oxygen Effect Observed in Certain Rad Mutants of Saccharomyces-Cerevisiae after Exposure to High-Energy ElectronsInternational Journal of Radiation Biology. 655-664.


Year Title
2010 Nanopores: A distinct transmembrane passageway in electroporated cellsAdvanced Electroporation Techniques in Biology in Medicine. Boca Raton, FL: CRC Press.

Research Overview

  • The current research is centered on understanding and characterization of cell response to pulsed electric fields treatments, with particular emphasis on nanosecond-duration pulses and cell death mechanisms.Main topics of the research include:1) mechanisms of the PEF-triggered cell death, characterization of the cell death pathways2) escalation of cell sensitivity to pulsed electric field (PEF) for advancement of PEF-mediated tissue and tumor ablation3) PEF-induced communication between the cells
  • Education And Training

  • Ph.D. in Radiation Biology/ Biophysics, Medical Radiology Research Center 1995
  • M.S. in Microbiology, Moscow State University 1982
  • Full Name

  • Olga Pakhomova