Overview

  • BS in Zoology and PhD in cancer studies at the University of Hull and University of Birmingham, England. Fellowships at the University of Birmingham, and Oxford, England, before coming to EVMS in 1997.Moved to the Center for Bioelectrics ODU in 2007.
  • Selected Publications

    Articles In Academic Journals

    Year Title
    2018 A Distributed-Deflection Sensor With a Built-In Probe for Conformal Mechanical Measurements of Costal Cartilage at Its Exterior SurfaceIEEE Sensors Journal. 822-829.
    2018 Dielectric properties of isolated adrenal chromaffin cells determined by microfluidic impedance spectroscopyBioelectrochemistry. 84-91.
    2017 Energy dissipation mapping of cancer cellsMicron. 24-29.
    2017 Nanosecond pulsed electric field induced changes in cell surface charge densityMicron. 45-49.
    2015 Presence and Localization of Pro- and Mature Forms of Biglycan and Decorin in Human Costal Cartilage Derived from Chest Wall Deformities. Austin J Musculoskelet Disord.  1012.
    2014 Dielectric Characterization of Costal Cartilage ChondrocytesBiochemical Biophysics Acta. -General Subjects. 146-152.
    2013 Atomic force microscopy characterization of collagen ’nanostraws’ in human costal cartilageMicron. 483-7.
    2013 Microfluidic Dielectric Spectroscopy of Costal Cartilage ChondrocytesBiophysical Journal. 674a-674a.
    2012 Decorin expression, straw-like structure, and differentiation of human costal cartilageConnect Tissue Res. 415-21.
    2012 Enhanced Killing Effect of Nanosecond Pulse Electric Fields on PANC1 and Jurkat Cell Lines in the Presence of Tween 80Journal of Membrane Biology. 611-616.
    2012 Probing nanoparticle interactions in cell culture mediaColloids Surf B Biointerfaces. 96-102.
    2011 Nanosecond pulse electrical fields used in conjunction with multi-wall carbon nanotubes as a potential tumor treatmentBiomed Mater. 011002.
    2011 Nanosecond pulse electrical fields used in conjunction with multi-wall carbon nanotudes as a potential tumor treatmentBiomed Mater. 011002.
    2011 Nanosecond pulse electrical fields used in conjunction with mutli-wall carbon nanotubes as a potential tumor treatment.Biomed Mater. 011002.
    2011 Nanosecond pulsed electric field induced cytoskeleton, nuclear membrane and telomere damage adversely impact cell survivalBioelectrochemistry. 131-4.
    2010 Dispersion state and toxicity of mwCNTs in cell culture medium with different T80 concentrationsColloids and Surfaces B-Biointerfaces. 36-43.
    2010 Variable number of tandem repeat polymorphisms (VNTRs) in the ACAN gene associated with pectus excavatumClinical Genetics. 502-4.
    2008 Cold atmospheric pressure air plasma jet for medical applicationsApplied Physics Letters.
    2007 Bioelectric effects of intense nanosecond pulsesIEEE Transact Dielectrics Electrical Insul. 1088-1109.
    2007 Compact, Nanosecond, High Repetition-Rate, Pulse Generator for Bioelectric StudiesIEEE Trans. Dielectrics and Electrical Insulation.
    2006 Family study of the inheritance of pectus excavatumJournal of Pediatric Surgery. 1699-703.
    2004 Increased risk for aplastic anemia and myelodysplastic syndrome in individuals lacking glutathione S-transferase genesPediatr Blood Cancer. 122-6.
    2004 Ultrashort electrical pulses open a new gateway into biological cellsProceedings of the Ieee. 1122-1137.
    2003 Differential effects in cells exposed to ultra-short, high intensity electric fields: cell survival, DNA damage, and cell cycle analysisMutat Res. 65-75.
    2002 A modified nick translation method used with FISH that produces reliable results with archival tissue sectionsMolecular Biotechnology. 257-60.
    2002 Fluorescence in situ hybridization on sperm using alkaline denaturationBiotechniques. 266-7.
    2001 Genetic aberrations of NAT2 and chromosome 8: Their association with progression in transitional cell carcinoma of the urinary bladderUrologia Internationalis. 235-239.
    1999 Aneuploidy frequencies in semen fractions from ten oligoasthenoteratozoospermic patients donating sperm for intracytoplasmic sperm injectionFertility and Sterility. 472-478.
    1999 Arylamine N-acetyltransferase type 2 (NAT2), chromosome 8 aneuploidy, and identification of a novel NAT1 cosmid clone: an investigation in bladder cancer by interphase FISHGenes Chromosomes Cancer. 376-83.
    1999 Genes for human arylamine N-acetyltransferases in relation to loss of the short arm of chromosome 8 in bladder cancerPharmacogenetics. 1-8.
    1999 Nuclear receptor co-repressor gene localizes to 17p11.2, a frequently deleted band in malignant disordersGenes Chromosomes Cancer. 191-3.
    1998 Characterisation of arylamine N-acetyltransferase in the urothelial cell line RT112Pathogenesis. 99-105.
    1998 Genetic alterations of N-acetyl transferase in transitional cell carcinoma of the bladderBritish Journal of Cancer. 154-154.
    1997 Human T-cell receptor zeta chain gene Map position 1q23.1Chromosome Res. 279.
    1997 Mapping AAC1, AAC2 and AACP, the genes for arylamine N-acetyltransferases, carcinogen metabolising enzymes on human chromosome 8p22, a region frequently deleted in tumoursCytogenet Cell Genet. 290-5.
    1996 Accelerated telomere shortening in ataxia telangiectasiaNat Genet. 350-3.
    1996 Arylamine N-acetyltransferase as a potential biomarker in bladder cancer: Fluorescent in situ hybridization and immunohistochemistry studiesBiomarkers. 55-61.
    1995 Does junk DNA regulate gene expression in humans?Clin Mol Pathol. M118-23.
    1995 FISH analysis on spontaneously arising micronuclei in the ICF syndromeJ Med Genet. 502-8.
    1993 A family showing no evidence of linkage between the ataxia telangiectasia gene and chromosome 11q22-23J Med Genet. 135-40.
    1993 Epidermal mosaicism and Blaschko’s linesJ Med Genet. 752-5.
    1992 Development of T-cell leukaemia in an ataxia telangiectasia patient following clonal selection in t(X;14)-containing lymphocytesLeukemia. 961-6.
    1992 Rapid interphase FISH diagnosis of trisomy 18 on blood smearsLancet. 495.
    1990 Rearrangement of the same chromosome regions in different SV40 transformed human skin keratinocyte lines is associated with tumourigenicityOncogene. 727-39.
    1989 Cultured skin keratinocytes from both normal individuals and basal cell naevus syndrome patients are more resistant to gamma-rays and UV light compared with cultured skin fibroblastsInt J Radiat Biol. 45-58.
    1987 Variant forms of ataxia telangiectasiaJ Med Genet. 669-77.

    Books

    Year Title
    2013 Detection of cytogenetic abnormalities in sperm from infertile males undergoing intracytoplasmic sperm injection.  New York: Humana Press.

    Chapters

    Year Title
    2011 Subcellular Biological Effects of Nanosecond Pulsed Electric FieldsPlasma for Bio-decontamination, Medicine and Food Security,”. NATO Science for Peace and Security Series, Springer Publishing.

    Conference Papers

    Year Title
    2002 Paternal origin of Y-chromosome-bearing cells in microchimeric nulligravid females with autoimmune disease.  491-491.
    2001 Glutathione S-transferase theta and mu (GSTM1 and GSTT1) gene expression in patients with aplastic anemia and myelodysplastic syndrome..  250-250.
    2001 Microchimerism for Y-chromosome bearing cells in nulligravid females presenting with pediatric autoimmune disease..  663-663.
    1999 Aneuploidy in sperm from fifty-four oligoasthenoteratozoospermic patients undergoing intracytoplasmic sperm injection..  A353-A353.
    1999 Genomic instability in bone marrow failure syndromes: High frequency of aneuploidy by fluorescence in situ hybridization and identification of a spindle checkpoint gene mutation..  674A-674A.
    1999 Glutathione-S transferase (GSTT1 and GSTM1) null genotypes in patients with aplastic anemia and myelodysplastic syndromes.  675A-675A.
    1999 Increased frequency of glutathione-S transferase (GSTT1) null genotype in patients with aplastic anemia and myelodysplastic syndromes..  A324-A324.

    Research Overview

  • Research interests.For more detail, see the Mike Stacey's Homepage tab.A basis tenant of biology is that normal differentiated cells will respond to their environments. Chondrocytes are no different, and the biomechanical and bioelectrochemical fluxes they experience result in the production of an extra cellular matrix able to withstand variable and extreme mechanical loads. To confound this, cartilage does not have a blood supply and cells experience, in fact require, hypoxic and acidic conditions to fully function. These factors all play a role in the poor ability of cartilage to regenerate. The regenerative ability of cartilage to restore itself is of great interest in sports related injuries.The biological/biomechanical/bioelectrochemical aspects of cartilage and how these interact in disease and injury reveals intriguing and multidisciplinary avenues of research. The membrane is where the cell interacts with the environment and specialized structures link the extra cellular matrix to the cells cytoskeleton and nucleus influencing gene expression. Our investigations examine the growth of chondrocytes in 3D under hypoxic conditions, changes in gene expression of membrane associated proteins, particularly those responsible for ion movement across membranes, the ion and hemi channels.The bioelectrochemistry of chondrocytes is of huge importance and is the focus of our research through a grant from the NIAMS. A microfluidic approach enables dielectric measurements of cells to be made, with correlation to ion channel and matrix gene expression. The measured impedance is modeled using a combination of physical models, such as Cole-Cole, Constant Phase Angle, Maxwell Wagner mixture, double shell models. Subcellular dielectric parameters, such as conductance and capacitance of the cell membrane and nuclear envelope, and conductivity of the cytoplasm and nucleoplasm, are obtained as a result of dielectric modeling.
  • Expertise Keywords

  • Biology : Biomedical sciences, Cell, Molecular Biology
  • Education And Training

  • Ph.D. in Cancer Biology, University of Birmingham 1989
  • Full Name

  • Michael Stacey