• Dr. Lee is a well-established interdisciplinary researcher and educator in the areas of Biochemistry & Bioenergetics, Biofuels & Biochar, and Water & Energy Environmental Sustainability Research.
  • Selected Publications

    Articles In Academic Journals

    Year Title
    2018 Experimental Determination of Proton-Cation Exchange Equilibrium Constants at Water-Membrane Interface Fundamental to BioenergeticsWater. 116.
    2018 Surface oxygenation of biochar through ozonization for dramatically enhancing cation exchange capacityBioresources and Bioprocessing. 1.
    2016 Biochar-surface oxygenation with hydrogen peroxideJournal of Environmental Management. 17-21.
    2016 Characterization of biochars produced from peanut hulls and pine wood with different pyrolysis conditionsBioresources and Bioprocessing. 15.
    2016 Experimental Demonstration of Localized Excess Protons at a Water-Membrane Interface and the Effect of other Cations on Their StabilityThe FASEB Journal. 634-1.
    2016 Investigation into the sources of biochar water-soluble organic compounds and their potential toxicity on aquatic microorganismsACS Sustainable Chemistry & Engineering. 2550-2558.
    2016 Toxicological study of biofuel ethanol with blue green alga Spirulina platensisAlgal Research. 110-115.
    2015 Proton-Electrostatic Localization: Explaining the Bioenergetic Conundrum in Alkalophilic BacteriaBioenergetics. 2.
    2014 Biochar from woody biomass for removing metal contaminants and carbon sequestrationJ. Ind. Eng. Chem. (Amsterdam, Neth.). Ahead of Print.
    2014 Comparative analysis of pinewood, peanut shell, and bamboo biomass derived biochars produced via hydrothermal conversion and pyrolysisJournal of Environmental Management. 303-308.
    2013 Colorimetric assay for quantitative analysis of butanol and other higher alcoholsABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY.
    2013 Comparative analysis of biochars produced via hydrothermal conversion and slow pyrolysisABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY.
    2013 Experimental bioenergetics study: Electrostatically localized protonsABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY.
    2013 Membrane surface charges attracted protons are not relevant to proton motive forceBioenerg.: Open Access. 1000e114/1-1000e114/3, 3 pp..
    2013 Molecular Characterization of Inhibiting Biochar Water-Extractable Substances Using Electrospray Ionization Fourier Transform Ion Cyclotron Resonance Mass SpectrometryEnvironmental Science & Technology. 13294-13302.
    2013 Potential Impact of Biochar Water-Extractable Substances on Environmental SustainabilityACS Sustainable Chemistry & Engineering. 118-126.
    2013 Synthetic Biology: A New Opportunity in the Field of Plant Biochemistry & PhysiologyJ Plant Biochem Physiol.
    2013 Tolerance of arthrospira platensis to biofuel alcoholsABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY.
    2012 Proton-electrostatics hypothesis for localized proton coupling bioenergeticsBioenerg.: Open Access. 1000104/1-1000104/9, 9.
    2010 Characterization of Biochars Produced from Cornstovers for Soil AmendmentEnviron. Sci. Technol.. 7970-7974.
    2010 James Weifu Lee, Robert S. Foote (Eds.): Micro and nano technologies in bioanalysis. Methods and protocolsAnalytical and bioanalytical chemistry. 1371-1372.
    2010 Sustainability: the capacity of smokeless biomass pyrolysis for energy production, global carbon capture and sequestrationEnergy Environ. Sci.. 1695-1705.
    2009 Micro and nano technologies in bioanalysisHumana Press.
    2007 Nanoelectrode-gated detection of individual molecules with potential for rapid DNA sequencingSolid State Phenomena. 1379-1386.
    2007 Rapid DNA sequencing by direct nanoscale reading of nucleotide bases on individual DNA chainsPerspectives in Bioanalysis. 245-263.
    2007 Single-strand DNA molecule translocation through nanoelectrode gapsNanotechnology. 424018.
    2007 Toward electronic conductance characterization of DNA nucleotide basesSolid State Phenomena. 1387-1390.
    2005 Economical CO 2, SO x, and NO x capture from fossil-fuel utilization with combined renewable hydrogen production and large-scale carbon sequestrationEnergy. 2558-2579.
    2005 Imaging nanometer metallocatalysts formed by photosynthetic deposition of water-soluble transition-metal compoundsJ Phys Chem B. 5409-13.
    2004 Interfacial photoredox molecular interactions: A new class of Hill reagents for Photosystem II reaction centersThe Journal of Physical Chemistry B. 3935-3939.
    2004 The Utilization of CO2 for the Creation of a Valuable and Stable Carbon Co? Product From Fossil Fuel Exhaust ScrubbingPapers of the American Chemical Society. Washington DC, USA: American Chemical Society. U1093.
    2004 Valuable and stable carbon co-product from fossil fuel exhaust scrubbingPrepr. Pap.-Am. Chem. Soc., Div. Fuel Chem. 352.
    2003 A new oxygen sensitivity and its potential application in photosynthetic H 2 productionApplied Biochemistry and Biotechnology. 303-313.
    2003 Effects of Root-Zone Warming on Water and Nutrient Uptake, and Photosynthesis of Cucumber Grown in a GreenhouseJournal of the Korean Society for Horticultural Science.
    2003 Integration of fossil energy systems with CO 2 sequestration through NH 4 HCO 3 productionEnergy Conversion and Management. 1535-1546.
    2003 Molecular Photovoltaics and Surface Potentials at the Air- Water InterfaceThe Journal of Physical Chemistry B. 14225-14230.
    2003 Removal of carbon dioxide from flue gas by ammonia carbonation in the gas phaseEnergy & Fuels. 69-74.
    2002 A new oxygen sensitivity in photosynthetic H2 productionPrepr. Symp. - Am. Chem. Soc., Div. Fuel Chem.. 761-765.
    2002 Improvement of photosynthetic CO2 fixation at high light intensity through reduction of chlorophyll antenna sizeAppl Biochem Biotechnol. 37-48.
    2002 SESSION 1-ADVANCES IN BIOMASS PRODUCTION AND PROCESSING-Improvement of Photosynthetic CO2 Fixation at High Light Intensity Through Reduction of Chlorophyll Antenna SizeApplied Biochemistry and Biotechnology-Part A-Enzyme Engineering and Biotechnology. 37-48.
    2001 Electrostatic potentials measurement of single photosynthetic reaction center by kelvin force microscopyProceedings of SCANNING 2001. 130.
    2001 Nanoscale Photosynthesis: Photocatalytic Production of Hydrogen by Platinized Photosystem I Reaction Centers¶Photochemistry and photobiology. 630-635.
    2001 PHOTOSYNTHESIS AND PHOTOCONVERSION-Nanoscale photosynthesis: Photocatalytic production of hydrogen by platinized photosystem 1 reaction centersPhotochemistry and photobiology. 630-635.
    2001 Solar photobiochemistry: Simultaneous photoproduction of hydrogen and oxygen in a confined bioreactorJ. Phys. Chem. B. 3605-3609.
    2000 Measurement of electrostatic potentials above oriented single photosynthetic reaction centersThe Journal of Physical Chemistry B. 2439-2443.
    2000 Microalgae: a green source of renewable H(2)Trends Biotechnol. 506-11.
    1999 Photosynthetic hydrogen and oxygen production by green algaePrepr. Symp. - Am. Chem. Soc., Div. Fuel Chem.. 851-854.
    1999 Programmable nanometer-scale metal bonding to single molecules by electrolytic deposition and depletionInvention Disclosure.
    1998 Molecular ionic probes: a new class of Hill reagents and their potential for nanofabrication and biometallocatalysisThe Journal of Physical Chemistry B. 2095-2100.
    1997 A new perspective on hydrogen production by photosynthetic water splittingACS Symp. Ser.. 209-222.
    1997 Biomolecular electronics: vectorial arrays of photosynthetic reaction centersPhysical Review Letters. 3294.
    1997 CO2 fixation and photoevolution of H2 and O2 in a mutant of Chlamydomonas lacking photosystem I. [Erratum to document cited in CA123:165257]Nature (London). 808.
    1997 Correction CO2 fixation and photoevolution of H2 and O2 in a mutant of Chlamydomonas lacking photosystem INature. 808-808.
    1997 Oxygenic Photoautotrophic Growth and Photosystem I: ResponseSCIENCE-NEW YORK THEN WASHINGTON-. 996-996.
    1997 Oxygenic photoautotrophic growth and photosystem I. Reply to commentsScience (Washington, D. C.). 996.
    1997 Photosystem-I Measurements in Mutants B4 and F8 of ChlamydomonasScience. 166-167.
    1996 New photosynthesis or old? ReplyNature (London). 305.
    1996 Oxygenic photoautotrophic growth without photosystem IScience. 364-367.
    1996 Platinization: a novel technique to anchor photosystem I reaction centres onto a metal surface at biological temperature and pHBiosensors and Bioelectronics. 375-387.
    1995 Bioelectronics and biometallocatalysis for production of fuels and chemicals by photosynthetic water splittingAppl. Biochem. Biotechnol.. 295-305.
    1995 CO2 fixation and photoevolution of H2 and O2 in a mutant of Chlamydomonas lacking photosystem INature (London). 438-41.
    1995 Chemical platinization and its effect on excitation transfer dynamics and P700 photooxidation kinetics in isolated photosystem IBiophysical Journal. 652-659.
    1995 Molecular electronics of a single photosystem I reaction center: studies with scanning tunneling microscopy and spectroscopyProceedings of the National Academy of Sciences. 1965-1969.
    1995 Temperature effect on production of hydrogen and oxygen byChlamydomonas cold strain CCMP1619 and wild-type 137cApplied Biochemistry and Biotechnology. 379-386.
    1994 Photosynthetic water splitting: in situ photoprecipitation of metallocatalysts for photoevolution of hydrogen and oxygenEnergy & Fuels. 770-773.
    1992 Quenching of chlorophyll excited states in photosystem I by quinones. Stern-Volmer analysis of fluorescence and photochemical yieldJ. Lumin.. 79-89.


    Year Title
    2013 Advanced biofuels and bioproducts.  Springer.
    2009 Micro and nano technologies in bioanalysis: methods and protocols.  New York: Humana Press.
    2003 Proceedings of the Twenty-Fourth Symposium on Biotechnology for Fuels and Chemicals held 28 April-1-May 2002 in Gatlinburg, Tennessee.  Humana Press Inc.
    1993 Excitation transfer dynamics and P700 photooxidation kinetics in isolated photosystem I.  Cornell University, May.


    Year Title
    2013 Biochar Fertilizer for Soil Amendment and Carbon SequestrationAdvanced Biofuels and Bioproducts. Springer New York.
    2013 Designer Photosynthetic Organisms for Photobiological Production of Ethanol from Carbon Dioxide and WaterAdvanced Biofuels and Bioproducts. Springer New York.
    2013 Designer Transgenic Algae for Photobiological Production of Hydrogen from WaterAdvanced Biofuels and Bioproducts. Springer New York.
    2013 Introduction: An Overview of Advanced Biofuels and BioproductsAdvanced Biofuels and Bioproducts. Springer.
    2013 Oxygenation of biochar for enhanced cation exchange capacityAdvanced Biofuels and Bioproducts. Springer.
    2013 Smokeless biomass pyrolysis for producing biofuels and biochar as a possible arsenal to control climate changeAdvanced Biofuels and Bioproducts. Springer.
    2013 Synthetic biology for photobiological production of butanol and related higher alcohols from carbon dioxide and waterAdvanced Biofuels and Bioproducts. Springer.
    2004 Algal H 2-Production Systems: Creation of Designer Alga for Efficient and Robust Production of H 2FY 2004 progress report. Oak Ridge National Laboratory Oak Ridge, TN.

    Conference Papers

    Year Title
    2018 Biochar surface oxygenation through ozonization for enhanced cation exchange capacity
    2018 Comparative study of wet vs dry biochar ozonization on oxygenation of biochar surface and dissolved organic carbon
    2018 Demonstration of horizontal gene transfer from genetically engineered cyanobacteria to wild-type E. coli
    2018 Experimental determination of proton-cation exchange equilibrium constants fundamental to bioenergetics
    2018 Proton motive force computation revealing latent heat utilization by localized protons at a liquid-biomembrane interface
    2018 Turning un-hydrolyzed cellulosic lignin residue into surface-oxygenated biochar through hydrothermal carbonization and wet ozonization
    2009 Experimental study on the ground support conditions of pipe ends in single span pipe greenhouse.  259-268.
    2006 Molecular Dynamics of DNA Translocation through Nanoelectrode Gaps
    2004 Development of sustainable fertilizer with positive local and global impacts
    2004 Stable biomass carbon co-products for fossil fuel exhaust scrubbing.  U675-U676.
    2003 Overcoming nation’s roadblocks to photosynthetic H2 production.  4-6.
    2002 Biomolecular optoelectronic devices and their application to artificial sight.  496-498.
    2002 Functional 3D nanoscale imaging of a single-molecule photovoltaic structure.  42A-42A.
    2002 Nanoscale photosynthesis, the photophysics of neural cells, and artificial sight.  83-85.
    2002 Oxygen sensitivity in algal H2 production.  U579-U580.
    2001 A novel strategy for CO2 sequestration and clean air protection
    2001 Application of photosynthesis to artificial sight.  4089-4091.
    2001 Nanoscale Optoelectronic Photosynthetic Devices
    2000 Molecular Electronics with Photosystem I Reaction Center Complex System
    1999 Biomolecular electronic devices with Photosystem I reaction centers.  62-63.
    1998 A study of photosynthetic reactions in PS I-deficient mutants of C-reinhardtii..  A16-A16.
    1998 Renewable hydrogen production by photosynthetic water splitting.  61.
    1997 A quantitative test of the”leaky”Z-scheme of photosynthesis..  MAME2-MAME2.
    1997 Hydrogen and oxygen production in mutant Fud26 of Chlamydomonas reinhardtii.  1-10.
    1996 Discovery of a new photosynthetic pathway: photosystem II light reaction can support complete photosynthesis.  MAMG1-MAMG1.

    Research Overview

  • Advanced Biochar Chemistry, Synthetic Biology, Advanced Biofuels, and Bioenergetics Research
  • Expertise Keywords

  • Biotechnology : Advanced Biochars Chemistry, Biofuels with Synthetic Biology, and Bioenergetics
  • Education And Training

  • Ph.D. in Photosynthesis Research and Physical Chemistry, Cornell University 1993
  • M.S. in Plant Physiology and Biochemistry, Cornell University 1988
  • Full Name

  • James Lee